Here are the 33 US AI startups that have raised $100M or more in 2025
The U.S. AI startup scene is booming in 2025. Funding momentum from 2024 has carried over, and this year has already seen multiple billion-dollar rounds and mega-deals. Here are the 33 US AI startups that have raised $100M or more in 2025, proving just how competitive the space has become.
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In 2024, 49 startups crossed the $100 million threshold, with seven securing billion-dollar rounds. As we near the end of Q3 in 2025, the numbers are already looking just as strong — if not stronger.
Let’s break down the major funding wins from this year’s standout AI companies.
August 2025: Fresh Momentum in AI
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EliseAI – Raised $250 million in a Series E, valuing the healthcare and housing automation platform at $2.2 billion. The round was led by Andreessen Horowitz.
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Decart – An AI research lab that secured $100 million at a $3.1 billion valuation, backed by Sequoia Capital, Benchmark, and Zeev Ventures.
July 2025: Healthcare and Generative AI Surge
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Fal – A generative media platform, closed a $125 million Series C led by Meritech Capital Partners, with Salesforce Ventures, Shopify Ventures, and Google AI Futures Fund also participating.
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Ambience Healthcare – Building an AI healthcare operating system, raised $243 million in a Series C led by Oak HC/FT and Andreessen Horowitz, with backing from Kleiner Perkins and OpenAI Startup Fund.
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Reka AI – Secured $110 million in a Series B, including investments from Snowflake and Nvidia, valuing the company at $1 billion.
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Thinking Machines Lab – Made headlines with a $2 billion seed round, one of the largest in AI history. Investors included Andreessen Horowitz, Nvidia, Accel, and AMD, valuing the startup at $12 billion.
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OpenEvidence – Raised $210 million at a $3.5 billion valuation for its AI-powered clinical search tool. The Series B closed on July 15.
Why These AI Rounds Matter
The fact that 33 US AI startups have raised $100M or more in 2025 highlights how central AI has become to healthcare, automation, and generative technologies. Investors are not just funding ideas — they are backing platforms that could reshape entire industries.
The rise of billion-dollar “mega-rounds” shows that AI is moving from experimentation to large-scale adoption. From diagnostic tools for clinicians to next-gen media creation, these startups are setting the pace for the global AI race.
2025 is shaping up to be another record-breaking year for AI in the U.S. With valuations soaring and new unicorns being minted every quarter, the competition is only heating up.
As we move into Q4, the key question is not whether more startups will raise $100M+, but which ones will cross the billion-dollar funding milestone next.
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